{"id":138,"date":"2022-02-20T18:44:21","date_gmt":"2022-02-20T18:44:21","guid":{"rendered":"http:\/\/localhost\/kpv\/?p=138"},"modified":"2025-02-13T12:52:43","modified_gmt":"2025-02-13T12:52:43","slug":"the-greactions","status":"publish","type":"post","link":"https:\/\/dalmolingroup.imd.ufrn.br\/kpv\/the-greactions\/","title":{"rendered":"The gReactions"},"content":{"rendered":"\n

To enhance the accuracy and clarity of the network representation, we introduce a new entity, called gReaction<\/strong>, which serves as the node in the primary graph G. The gReaction<\/strong> is defined by associating principal substrates and products, along with incorporating information regarding the reversibility of reactions. This approach ensures a more precise characterization of enzymatic activities within the metabolic network.<\/p>\n\n\n\n

To illustrate this method, we examine a segment from the initial steps of the Glycolysis\/Gluconeogenesis pathway (KEGG map 00010)<\/em><\/a>, focusing on substrates and products such as \u03b1-D-Glucose, \u03b1-D-Glucose 6P, \u03b2-D-Glucose, and \u03b2-D-Glucose 6P (Figure A). The schematic representation in Figure B highlights five distinct groups of reactions involved in this process:<\/p>\n\n\n\n

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  1. Four enzymes catalyzing three reactions<\/strong> to convert \u03b1-D-Glucose into \u03b1-D-Glucose 6P.<\/li>\n\n\n\n
  2. The same four enzymes<\/strong> {2.7.1.1, 2.7.1.2, 2.7.1.63, 2.7.1.147} mediating three different reactions<\/strong> to convert \u03b2-D-Glucose into \u03b2-D-Glucose 6P.<\/li>\n\n\n\n
  3. Two enzymes<\/strong> facilitating the interconversion reaction<\/strong> between \u03b1-D-Glucose 6P and \u03b2-D-Glucose 6P.<\/li>\n\n\n\n
  4. Two additional reactions<\/strong>, each mediated by a single, distinct enzyme.<\/li>\n<\/ol>\n\n\n\n

    This pathway segment presents two primary challenges in the construction of graph G:<\/p>\n\n\n\n